Taqman Real-Time PCR Detects Avipoxvirus DNA in Blood of Hawaìi `Amakihi (Hemignathus virens)
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منابع مشابه
Taqman Real-Time PCR Detects Avipoxvirus DNA in Blood of Hawaìi `Amakihi (Hemignathus virens)
BACKGROUND Avipoxvirus sp. is a significant threat to endemic bird populations on several groups of islands worldwide, including Hawai'i, the Galapagos Islands, and the Canary Islands. Accurate identification and genotyping of Avipoxvirus is critical to the study of this disease and how it interacts with other pathogens, but currently available methods rely on invasive sampling of pox-like lesi...
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Lesions resembling knemidokoptic mange on the feet and tarsometatarsi of two Hawai'i' Amakihi (Hemignathus virens) were observed while the researchers were mist-netting wild passerines at Manuka Natural Area Reserve on the island of Hawai'i between 14 June 2007 and 19 June 2007. During subsequent mist-netting from September 2007 through February 2008, we found 26% (7/27) of the Hawai'i' Amakihi...
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The polymerase chain reaction (PCR), first described in 1985, is a highly sensitive and specific technique used for the detection of nucleic acids [55]. The inventor of this technology earned a Nobel prize for his achievement [43,44], which has revolutionised research and diagnostic possibilities. Qualitative PCR is a well established and straightforward technology, but quantification of specif...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0010745